Antioxidant activity of transgenic chicory plants with interferon-α2b gene.
Keywords:
antioxidant activity, genetic transformation, Cichorium intybus L., human interferon-α2bAbstract
Antioxidant activity of transgenic chicory plants with interferon-α2b gene was investigated. The antioxidant activity of nine plant lines from ten investigated varied from 11,2±0,69 х10-6 to 23,30±0,97 х10-6 mg/ml min and was higher than activity of control nontransformed plants (8,8±1,14 х10-6 mg/ml min). Direct relation between the presence of ifn-α2b gene reverse transcripts and interferon-like activity with increasing of antioxidant activity was not found. The increase of antioxidant activity may be caused by genetic transformation - transferring of the foreign gene in plants.References
Enikeev A. G. Agrobacterial transformation as complex biotical stressing factor / A. G. Enikeev, T. V. Kopytina, L. A. Semenova, et al. // Journal of stress Physiology and Biochemistry. – 2008. – Vol. 4., No 1. – P. 11-19.
Колупаев Ю. Е. Антиоксидантная система растений: участие в клеточной сигнализации и адаптации к действию стрессоров / Ю. Е. Колупаев, Ю. В. Карпец, А. И. Обозный // Вісник Харківського національного університету Серія Біологія. – 2011. – Вип. 1, № 22. – С. 6-34.
Foyer C. H. Protection against oxygen radicals: an important defense mechanism studied in transgenic plants // C. H. Foyer, P. Descourvierse, K. J. Kunert // Plant Cell Environ. – 1994. – Vol. 17, No 5. – P. 507-523.
Asada K. The water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons / K. Asada // Annual Review of Plant Physiology and Plant Molecular Biology. – 1999. – Vol. 50. –. 601-639
Gossett D. G. Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton / D. G. Gossett, E. P. Millhollon, M. C. Lucas // Crop Sci. – 1994. –Vol. 34, No 3. – P. 706-714.
Olmos E. Induction of severa1 antioxidant enzymes in the selection of a salt-tolerant cell line of Pisum sativum. / E. Olmos, J. A. Hernandez, F. Sevilla, E. Hellin // J. Plant Physiol. – 1994. – Vol. 144, No 4. – P. 594-598.
Salt-induced oxidative stress in chloroplasts of pea plants / J. A. Hernandez, E. Olmos, F. J. Corpas et al. // Plant Sci. – 1995. – Vol. 105, No 2. – P. 151-167.
Lu G. Interferon-alpha enhances biological defense activities against oxidative stress in cultured rat hepatocytes and hepatic stellate cells / G. Lu , I. Shimizu , X. Cui et al // J. Med Invest. – 2002. – Vol. 49, No 3-4. – P. 172-81.
Перенесення гена біосинтезу інтерферону-α2b в рослини цикорію (Cichorium intybus L.) методом агробактеріальної трансформації / Н.А. Матвєєва, АМ. Шаховський, І.М Герасименко та ін. // Біополімери і клітина. – 2009. – Т.25, № 2. – С. 120-125.
Семенов В. Л. Метод определения антиокислительной активности биологического материала / В. Л. Семенов, А. М. Ярош // Украинский биохимический журнал. – 1985. – Т. 57, № 3. – С. 50-52.
Lavelli V. Antioxidant Activity of Minimally Processed Red Chicory (Cichorium intybus L.) Evaluated in Xanthine Oxidase-, Myeloperoxidase-, and Diaphorase-Catalyzed Reactions / V. Lavelli // J. Agric. Food Chem. – 2008. – Vol. 56, No 16. – P. 7194-7200.
Kim Y.-H. Expression of Arabidopsis NDPK2 increases antioxidant enzyme activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants / Y.-H. Kim, S. Lim, K.-S. Yang // Mol. Breeding. – 2009. – Vol. 24, № 3. – P. 233-244.
Yang L. Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2b, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana / L. Yang, R. Tang, J. Zhu // Plant Mol. Biol. – 2008. – Vol. 66, No 4. – P. 329–343.
Harper D. B. Mechanisms of paraquat tolerante in perennial ryegrass. Role of superoxide dismutase, catalase, and peroxidase / D. B. Harper, B. M. R. Harvey // Plant Cell Environ. – 1978. – Vol. 1, No. 3 – P. 211-215.
Wise R. R. Chilling-enhanced photooxidation. Evidence for the role of singlet oxygen and endogenous antioxidants / R. R. Wise, A. W. Naylor // Plant Physiol . 1987. – Vol. 83, No. 2. – P. 278-282.